X-α-Gal is a chromogenic substrate for α-galactosidase, yielding a blue precipitate. X-α-Gal is used for detecting two-hybrid interactions directly on the culture media. This substrate eliminates the need for β-galactosidase liquid and filter-lift assays in two-hybrid analysis.

X-α-Gal is commonly used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacilli species.

Product Specifications
X-α-Gal
5-Bromo-4-chloro-3-indoxyl-α-D-galactopyranoside
X-α-D-Galactoside

MOLECULAR BIOLOGY GRADE

Formula: C₁₄H₁₅BrClNO₆

MW: 408.64 g/mol

PubChem Chemical ID: 10173103

Storage/Handling:Store at -20°C. Protect from light.