DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca²⁺.

Product Specifications
DNase I
Deoxyribonuclease I
DNase I, Bovine Pancreas >2000 Kunitz U/mg

Unit Definition: One Kunitz Unit will produce an A₂₆₀ of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.